ARS | Publication request: RESTRICTION ENZYME ANALYSES OF CYTOPLASMIC DIVERSITY IN LEEK

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 18, 1996
Publication Date: N/A

Technical Abstract: Hybrid leek (Allium ampeloprasum) is significantly more uniform and higher yielding than open-pollinated populations. Because leek has perfect flowers, a male-sterility system is required to economically produce hybrid seed. No cytoplasmic male sterility (CMS) has been described in leek. Restriction fragment length polymorphisms (RFLPs) in the chloroplast and mitochondrial genome have correlated with the expression of CMS in many crops. We undertook restriction-enzyme analyses of the chloroplast and mitochondrial DNAs to identify polymorphic organellar genomes among 65 accessions of cultivated leek. Polymorphisms were detected both in the chloroplast and mitochondrial genomes. Reciprocal crosses were generated to establish the transmission of the organellar genomes of leek.