Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 7, 1996
Publication Date: N/A
Technical Abstract: Attenuation of Marek's disease virus by passage in tissue culture is well established. During the process of attenuation, several changes in the virus genome have been reported. However, it is not clear which change(s) on the genome is responsible for the attenuation. Differential display (dd) is a novel technique which utilizes RT-PCR with a short 5' primers and anchored oligo-dT 3' primers to compare expressed mRNAs in two different cell populations. We have applied this technology to the RNA from virulent Md11- and passage-attenuated Md11-infected chicken embryo fibroblast cells in order to detect differences in mRNA expression in these cells. So far, we have examined 104 pairs of primers (26 5' primers X 4 3' primers) for dd PCR and found four virulent virus-specific fragments. One of them was localized next to 132bp repeat region and may represent the difference in the transcriptional pattern reported between virulent and attenuated virus. Two clones showed homology to the 5' region of ICP4 homologue. The last clone showed partial homology with the Bam HI-D fragment.