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United States Department of Agriculture

Agricultural Research Service

Title: Efficacy of Two Avian Salmonella-Immune Lymphokines Against Liver Invasion in Chickens by Salmonella Serovars with Differing O-Group Antigens

Authors
item Ziprin, Richard
item Kogut, Michael
item Deloach, John

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 21, 1996
Publication Date: N/A

Interpretive Summary: Salmonella bacteria are well known as a cause of food-poisoning in humans. Efforts to reduce or eliminate these bacteria from poultry have included work on substances called immune lymphokines which are produced by poultry. These substances aid the natural defenses of young chickens. This work is part of a series of studies of immune lymphokines. We examined the activity of two immune lymphokines against three types of Salmonella and found that protection against organ invasion conferred by Salmonella-immune lymphokines is unrelated to certain characteristics of the immunizing bacteria. This information indicates that it is possible to use Salmonella-immune lymphokines for protection against different kinds of Salmonella. These data are an important piece of the total information needed to develop effective ways to reduce Salmonella contamination of poultry and eggs thus helping to assure that we continue to have a safe supply of poultry and poultry products.

Technical Abstract: Newly hatched chicks are susceptible to organ invasion by various serovars of Salmonella choleraesuis. We have previously shown that intraperitoneal administration of Salmonella choleraesuis serovar Enteritidis-immune lymphokines (SEILK) increases resistance to organ invasion by S. enteritidis (SE) in day-of-hatch chicks. In the present study we determined that Salmonella-immune lymphokines can provide protection against organ invasion by serovars that are unrelated to the original serovar used as an immunogen. Immune lymphokines were produced from the splenic T cells of mature hens immunized with either viable SE or S. chloleraesuis serovar Anatum (SA). Day-of-hatch chicks were treated with either SA-immune lymphokine (SAILK) or with SEILK and challenged per os with either S. choleraesuis serovar Typhimurium (ST) or SE one hour later. Liver samples were aseptically collected twenty hours later and were cultured to detect salmonellae. We found that SAILK conferred resistance against invasion by either SE, a serogroup D organism, or ST, a serogroup B organism. Furthermore, SEILK reduced organ invasion by ST. These observations together reveal that the protection against organ invasion conferred by Salmonella-immune lymphokines is unrelated to the serovar specific antigenic characteristics of the immunizing bacteria.

Last Modified: 9/10/2014
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