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United States Department of Agriculture

Agricultural Research Service

Title: Comparative Viability of Bovine Sperm Frozen on a Cryomicroscope on in Straws

Authors
item Penfold, L - ARS, LPSI, GGPL
item Garner, D - UNIVERSITY OF NEVADA
item Donoghue, Ann
item Johnson, Lawrence

Submitted to: Theriogenology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 24, 1996
Publication Date: N/A

Interpretive Summary: The accuracy and repeatability of freezing rates and effects of evaporation were examined using a new cryomicroscope system to establish its usefulness in assessing the development of cryopreservation protocols. Post-thaw sperm plasma membrane integrity, as assessed by using combinations of fluorescent stains and flow cytometry, was used in evaluating protocols for freezing sperm on the cryomicroscope. This study showed that sperm could be recovered after freezing on the cryomicroscope and establishes the Linkam BCS 196 as a useful tool for the study of sperm cryopreservation.

Technical Abstract: The accuracy and repeatability of freezing rates and effects of evaporation were examined using a new cryomicroscope system to establish its usefulness in assessing the development of cryopreservation protocols. Post-thaw sperm plasma membrane integrity, as assessed by using combinations of fluorescent stains and flow cytometry, was used in evaluating protocols for freezing sperm on the cryomicroscope. Semen were frozen in 0.5 ml straws, 0.25 ml straws or in a quartz crucible using a Linkam BCS 196 cryomicroscope. Samples frozen using the cryomicroscope contained 29 % plasma membrane intact sperm. Cryopreservation of sperm in 0.5 ml straws resulted in 22% plasma membrane intact sperm while sperm frozen in 0.25 ml straws resulted in 34% plasma membrane intact sperm. The ability to recover sperm after freezing on the cryomicroscope establishes the Linkam BCS 196 as a useful tool for the study of sperm cryopreservation.

Last Modified: 12/26/2014
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