Submitted to: Oilseed Processing Clinic Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 12, 1996
Publication Date: N/A
Technical Abstract: Phospholipids (PLs), sterols and tocopherols are important minor constituents of oilseeds. Commercial oil processing produces these substances as by-products for a wide variety of usage in food and pharmaceutical industries. Analyses of the compounds provide useful information on their compositional profiles affected by genetic modification of selected oilseeds. Compositions of the polar lipid classes/subclasses are known to have significant bearing on the chemical and physical properties of cell membranes. We report efficient analytical methods for the simultaneous separation and quantitation of the minor constituents of crude oils derived from soybeans and canola seeds. Enriched PL fractions, isolated from the oilseeds by hexane extraction and silica column chromatography, were analyzed by normal-phase and reversed high-performance liquid chromatography (HPLC) evaporative light scattering detection. Saponified sterol isolates were analyzed as their trimethylsilyl derivatives by capillary gas chromatography-flame ionization detection. Crude oil samples were analyzed directly for tocopherols by normal-phase HPLC-fluorescence detection. The compositional data for soybean and canola oils were compared. The impact of genetic breeding of oilseeds on the minor constituents has been assessed.