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United States Department of Agriculture

Agricultural Research Service

Title: Differentiation of Respprrs Vaccine from Field Strains of Porcine Reproductive and Respiratory Syndrome Virus by Rt-Pcr and Restriction Enzyme Analysis

item Wesley, Ronald
item Mengeling, William
item Lager, Kelly
item Landgraf, John - USDA, APHIS, NVSL, DVL
item Frey, Merwin - USDA, APHIS, NVSL, DVL

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Proceedings
Publication Acceptance Date: October 18, 1996
Publication Date: N/A

Technical Abstract: The source of porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) isolated from diagnostic samples is often in doubt so we have investigated the suitability of restriction enzyme analysis for differentiating strain RespPRRS from field strains of PRRSV. Based on the ORF 5 sequence of RespPRRS/VR 2332 and the other 22 strains of PRRSV included in this study, we hypothesize that 4 restriction enzyme gel patterns characterize the RespPRRS vaccine virus genotype with high assurance. This differential test will be a valuable tool in PRRS epidemiological studies and will be useful in identifying individual strains in cases of multi-strain PRRSV infections.

Last Modified: 3/29/2015
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