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Title: GLIADIN BLOCK ANALYSIS BY RP-HPLC AND CAPILLARY ELECTROPHORESIS FOR GENOTYPE IDENTIFICATION AND QUALITY PREDICTION

Author
item Bietz, Jerold
item WAGA, J - PLANT BREEDING INSTITUTE

Submitted to: International Gluten Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 9/6/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Gliadins are coded by genes at six complex loci on wheat chromosome groups 1 and 6. Genes in each locus are closely linked, causing co-expression of proteins they code. Alleles or "blocks" differ among varieties, so genotypes can be represented by alleles at each locus [Sozinov and Poperelya, Ann. Technol. Agric. 29:229-245 (1980)]. Gliadin blocks also indicate quality, so allele analysis is valuable for selection during breeding, and for genotype identification. Gel electrophoresis is most used to identify gliadin blocks, but reversed-phase high-performance liquid chromatography (RP-HPLC) and capillary electrophoresis (CE) offer advantages for gliadin analysis. We, therefore, used gel electrophoresis, RP-HPLC, and CE to analyze lines from a cross of wheat varieties Alcedo and Trakija, differing by single gliadin alleles. Each method revealed compositions and recombination of gliadin alleles, useful for quality selection during breeding. HPLC and CE, because of their excellent resolution, revealed some gliadins not apparent by gel electrophoresis. Such information may help extend knowledge of gliadin alleles. RP-HPLC and CE can become valuable methods, complementary to gel electrophoresis, for block analysis during wheat breeding and in genetic studies.