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United States Department of Agriculture

Agricultural Research Service

Title: Hplc-Evaporative Light Scattering Detection of Nitrogenous Phospholipids Ona Polyvinyl Alchol Column

Authors
item Abidi, Sharon
item Mounts, Timothy
item Rennick, Kathy

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: April 28, 1996
Publication Date: N/A

Technical Abstract: Nitrogenous phospholipids(PL's) are biologically essential substances occurring ubiquitously in cell membranes of animals and plants. PL compositional variations are known to have significant influence on the cellular biochemical processes. Analyses of the polar lipid species also provide useful information about the impact of genetic breeding on PL distributions in vegetable oils. Further, good analytical techniques for PL measurements have been of continuous interest to lipid scientists. We wish to report the development of a new reversed-phase HPLC-evaporative light scattering detection (ELSD) method for the simultaneous separation and quantitation of the molecular species of phosphatidylcholine (PC), phosphatidylethanolamine (PE), N-alkyl homologues of PE, and sphingomyelin derived from various sources. A polyvinyl alcohol column was used for these PL assays. Subcomponents or molecular species were resolved under isocratic elution with an acetonitrile-methanol-water mobile phase at a flow rate of 1 ml/min. There were distinct differences in HPLC component profiles between the HPLC-ELSD and HPLC-UV methods. The HPLC-ELSD results are discussed in the context of component resolution, detection sensitivity, and analytical utility. Application of the new HPLC-ELSD technique enabled the quantitative analysis of molecular species of several samples of PC and PE isolated from soybean and canola oils. The analytical data were compared with those obtained with a conventional reversed-phase method.

Last Modified: 8/27/2014
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