Submitted to: Pittsburg Conference on Analytical Chemistry and Applied Spectroscopy
Publication Type: Abstract Only
Publication Acceptance Date: March 3, 1996
Publication Date: N/A
Technical Abstract: Development of molecular imprinted polymers is a technology that utilizes functionalized polymers formed in the presence of a "print" molecular (the analyte of interest). Molecularly-imprinted polymers (MIPs) have been utilized as solid supports for high performance liquid chromatography (HPLC), for thing-layer chromatography, and for receptor assays (molecularly imprinted sorbent assays, MIA). These latter assays are formatted similar to an immunoassay. However, they are more resistant to organic solvent effects than are antibody molecules. Thus, they may be more useful for the analysis of highly lipophjilic compounds such as many pesticides, and toxic chemicals that must be extracted from the sample matrix with an organic solvent. We have developed molecularly imprinted polymers to the agrochemicals atrazine, salinomycin, and ceftiofur. We have investigated the use of these MIPs as reagents for the development of analyte specific MIAs. In the case of atrazine, specific binding was significantly influenced by the type of solvent used. Organic solvents resulted in increased binding of analytic to the atrazine MIP vs. a control polymer, polymerized without atrazine present. We also have investigated the use of MIPs as the solid phase for HPLC. Our results demonstrated that various s-triazines were selectively retained. We investigated the use of molecularly imprinted polymers as solid phase matrices for extraction and cleanup of biological sample extracts. These experiments demonstrate that compounds are present in crud extracts of beef tissue that inhibit the ELISA, but these interfering compounds could be readily removed using MISPE. Thus, MISPE, represents a rapid, inexpensive system for analysis of residues in complex biological matrices.