Submitted to: Electron Microscopy Society of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 1996
Publication Date: N/A
Technical Abstract: Salmonella enteritidis (SE) is of great concern to the poultry industry due to the organism's ability to penetrate the intestinal mucosa of the laying hen and subsequently colonize the ovaries and yolk membrane. Interference with the ability of the organism to invade has been linked to the activation and recruitment of inflammatory polymorphonuclear cells, heterophils, to the lamina propria of the intestinal tract. Activated isolated heterophils displayed increased receptor-mediated, CD11b/CD18, adherence in vitro to BSA-coated slides as compared to control heterophils from PBS-injected birds. In preparations of cells incubated with a BSA matrix examined by SEM, unstimulated control heterophils were sparse and of generally round or flattened morphology with few cellular projections while SE-ILK stimulated heterophils were evident at a greater population density and displayed increased numbers of filamentous projections of cell cytoplasm. SE-ILK stimulated heterophils which migrate to and were isolated from the peritoneal cavity of immunized birds exhibited increased in vitro attachment and a greater degree of cytoplasmic extension compared to the cells isolated from peripheral blood. These findings indicate that similar to other animal models, transendothelial migration of activated heterophils is an adhesion molecule mediated event specifically CD11b/CD18, and this activation can be discerned by morphological traits as demonstrated in this study.