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United States Department of Agriculture

Agricultural Research Service

Title: Production and Characterization of Monoclonal Antibodies to Microorganisms Contained in a Continuous Culture That Protects Chicks Against Salmonellae Colonization

item Young, Colin
item Nisbet, David
item Stanker, Larry
item Deloach, John

Submitted to: American Society of Microbiologists Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: February 26, 1996
Publication Date: N/A

Technical Abstract: Intestinal bacteria from adult chickens have been successfully grown under anaerobic continuous culture in chemostats. We have previously described such an anaerobic culture system (CF3) containing some 29 bacterial species. The administration of CF3 cultures to newly hatched chicks results in exclusion of salmonellae and is known as competitive exclusion. The exact mechanisms(s) responsible for control remains to be elucidated. In order to determine the protective capacity and/or fate of these individual bacteria in the bird, we have generated a panel of monoclonal antibodies against the constituent microorganism of mixed microflora CF3 culture. These monoclonal antibodies are being used to quantitate the number of specific bacteria in the ceca of chicks inoculated with CF3. In this study, we present data on the preparation and characterization of monoclonal antibodies to Fusobacterium spp., Bifidobacterium spp., Enterococcus avium, Propionobacteria granulosum, Eubacteria spp., and Veillonella spp. from the CF3 culture. Five to 10 monoclonal antibodies to each bacterial spp. were generated and the following characteristics for each antibody determined: Isotype (IgA, IgG1, IgG2a, IgG2b, IgM, IgG3, Kappa chain, Lambda chain); sensitivity (10**2-10**4 cfu); specificity (all were highly specific as judged by cross-reactivity to a panel of heterologous bacteria); antigen recognition by Western blot analysis. Furthermore, these monoclonal antibodies have been used to validate immunoassays for detecting bacterial spp. in the CF3 culture and in the ceca of chicks.

Last Modified: 4/18/2015