Submitted to: Avian Immunology Research Group Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 1995
Publication Date: N/A
Technical Abstract: Hematopoietic colony stimulating factors (CSF) regulate the growth and development of phagocytic cell progenitors and also augment functional activation of phagocytes. Granulocyte-CSF (G-CSF) is the CSF that acts specifically upon granulocyte progenitor cells and mature granulocytes. We have shown that lymphokines (ILK) from T cells of birds immunized against Salmonella enteritidis (SE) induce a granulocyte (PMN) inflammatory response in chicks challenged with SE. This inflammatory response was characterized by a) a dramatic peripheral blood granulopoiesis, b) a potentiation of the biological functions of the circulating PMNs, and c) a directed influx of these activated PMNs to the site of bacterial invasion. In the current study, we determined the presence of G-CSF in ILK by Western blot analysis using a goat polyclonal anti-human G-CSF antibody (Ab). Using this Ab, we then evaluated the role of G-CSF in the ILK-induced protective inflammatory response in chickens against SE. Pretreatment of ILK with the Ab totally abolished the colony-stimulating activity of the ILK. Furthermore, Ab treatment of ILK resulted in a) an elimination of the ILK-induced peripheral blood heterophilia with a dramatic inhibition of ILK-mediated protection against SE organ invasion and b) an elimination of accumulation of inflammatory rate of chicks challenged intraperitoneally with SE. Taken together, these studies demonstrate for the first time the contribution of G-CSF to PMN activation and the immunoprophylaxis of SE infection by ILK in neonatal chickens.