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United States Department of Agriculture

Agricultural Research Service

Title: A Semi-Selective Medium for Detecting Fusarium Solani, the Causal Organism of Soybean Sudden Death Syndrome

item Huang, Yi - UNIV OF ILLINOIS
item Hartman, Glen

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: July 27, 1996
Publication Date: N/A

Technical Abstract: A semi-selective medium was developed to induce blue pigmentation for detection of F. solani from infested soils and infected soybeans. A basal medium was used with different carbohydrates at different concentrations to induce blue pigmentation using a pure culture of the fungus. Of the carbohydrates tested, sucrose at 20 g/l produced the most consistent blue pigmentation. With the addition of quintozen and four antibiotics, the semi-selective medium was compared to Nash's medium using F. solani-infested soil with a range of 102 to 106 conidia/g added to pasteurized soil. The recovery rate from each medium was similar; however, the semi-selective medium induced blue colonies while Nash's medium did not. The semi-selective medium has been used to detect the fungus from infected roots and from field soil.

Last Modified: 4/22/2015
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