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Title: REDUCTION OF CYTOTOXICITY OF MONILIFORMIN IN CULTURED SKELETOMYOCYTES BY SUPPLEMENTATION OF GLUCOSE

Author
item WU, WEIDONG - UNIV OF WI, MADISON
item Vesonder, Ronald

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/12/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The cytotoxicity of moniliformin, a Fusarium mycotoxin, on cultured chicken embryonic skeletomyocytes observed in Medium 199, disappeared or reduced when these cells were cultured in Medium RPMI-1640, depending on the toxin levels. The concentrations of glucose and thiamin in Medium RPMI-1640 are 2x and 100x those in Medium 199, respectively. To find out which nutrient was responsible for the reduced cytotoxicity, glucose and thiamin levels were supplemented to Medium 199, individually and in combination, to levels comparable to those in Medium RPMI-1640. Only when the glucose level was increased in Medium 199 was the cytotoxic effect of moniliformin negated or reduced. Thiamin supplemented to Medium 199 offered no protection. Measurements of supernatants of cell cultures indicated that moniliformin accelerated the depletion of glucose and accumulation of lactate in a dose dependent manner. These observations indicate that cytotoxicity of moniliformin is related to glucose metabolism. Supplemental glucose for glycolytic formation of ATP would permit myocytes to survive under moniliformin insult.