Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 4, 1996
Publication Date: N/A
Technical Abstract: Leuconostoc mesenteroides B-21297 is a mutant of L. mesenteroides B-1355 that is essentially free of dextransucrase activity and produces a two-fold increase in the alternansucrase activity. The purification of the extracellular alternansucrase activity from B-21297 incorporated the use of a novel alternanase enzyme to partially degrade endogenous alternan, which significantly enhanced the purification of the alternansucrase by ion exchange chromatography. Electrophoretic analysis of the purified enzyme showed a series of enzymatically active bands that range from 135 to 145 kDa in molecular weight. Preparative electrophoresis was used to prepare individual protein bands for protein microsequencing analysis.