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United States Department of Agriculture

Agricultural Research Service

Title: Chicken Mhc Class I Specific Alloantisera Induced by Cloned Bfiv Sequences

item Hunt, Henry
item Bacon, Larry

Submitted to: Poultry Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: July 8, 1996
Publication Date: N/A

Technical Abstract: Chicken MHC class I (BFIV) sequences were inserted into an avian retrovirus (RCAS) and expressed in an avian lymphoblastoid cell line (RP9). The transfected BFIV-expressing cell lines were used as an immunogen and resulted in the production of class I specific alloantisera. The class I sequences were obtained from BFIV12, BFIV13 and BFIV21 alleles. The alloantisera produced were tested by hemagglutination with cells from MHC congenic lines of haplotypes B**2, B**5, B**12, B**13, B**15, B**19 and B**21. Agglutination was seen with both the BFIV12 and BFIV13 alloantisera with cells of the B**12 and B**13 haplotypes. BFIV21-induced antisera agglutinated cells from B**5 and B**21 haplotypes. These cross-reactions were predicted due to the nucleotide and subsequent amino acid sequence similarities between the BFIV12 and BFIV13 alleles and between the BFIV5 and BFIV21 alleles. These antisera are valuable in determining MHC class I haplotype without confounding BG antigenic differences.

Last Modified: 4/20/2015
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