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Title: EXTRACTION OF DNA FROM WOODY LEGUMES AND ANALYSIS BY RAPD-PCR

Author
item Pennington, Rodney
item ADAMS, ROBERT - PLANT BIOTECHNOLOGY CNTR
item Tischler, Charles
item Johnson, Hyrum
item Polley, Herbert
item Mayeux Jr, Herman
item Brown, Daniel

Submitted to: Plant Physiology Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 4/12/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Woody legumes have proliferated greatly on grasslands and savannas during the last 150 years. Investigation of patterns of genetic variation which may have contributed to this proliferation requires reliable methods for isolating and analyzing genomic DNA. We tested several protocols for extracting DNA from leaf material and analyzed extracted DNAs with the random amplified polymorphic DNA (RAPD)-PCR method. The following woody legumes from the southwestern U.S. were studied: Prosopis glandulosa var. glandulosa (honey mesquite), P. velutina (velvet mesquite), P. pubescens (screwbean), Acacia wrightii (catclaw), A. smallii (huisache), Parkinsonia aculeata (paloverde), and Gleditsia triacanthose (honey locust). A cetyltrimethylammonium bromide-based method produced readily amplifiable DNA from immature and mature leaf material of all species examined except A. smallii. Amplifiable DNA from A. smallii was obtained with a more laborious method based on differential precipitation of nucleic acids and carbohydrates with 2-butoxyethanol. RAPD-based characterizations of DNA proved effective in discerning inter- and intra-specific differences among these woody legumes.