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United States Department of Agriculture

Agricultural Research Service

Title: Protective Effects of Supernatants from Virally Transformed Spleen Cells from Salmonella Enteritidis Hyperimmunized Chickens

Authors
item Moyes, Rita
item Droleskey, Robert
item Deloach, John
item Kogut, Michael

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 15, 1995
Publication Date: N/A

Technical Abstract: It has been previously demonstrated that immunoprophylactic administration of Salmonella enteritidis-immune lymphokines (SE-ILK) confers increased resistance to Salmonella enteritidis (SE) organ invasion in day-old chicks. The increased resistance is associated with an inflammatory cell influx consisting mainly of activated heterophils which peaked at 4 hr after SE-ILK administration. The objective of this study was to find a method in which SE-ILK production could be scaled up for widespread poultry industry usage. Whole spleen cell suspensions from SE- hyperimmunized adult birds were Con A stimulated in vitro for 72 hr then virally transformed using the avian reticuloendotheliosis virus (REV). Fluorescence activated cell scanning (FACSCAN) analysis of the resulting transformed cells demonstrated that the lines were CD4+ (55 +/- 5%), CD8+ (30 +/- 5%), and Ig-. The resulting supernatant (VILK),when administered in conjunction with SE, induced a heterophilic influx that was significantly greater than SE alone (p<.001) and decreased organ invasion from 90 +/- 3.5% SE-organ culture positive (SE controls) to 9.7 +/- 6.3% positive (VILK treated) as compared to 11.57 +/- 10.8% positive (SE-ILK treated). The results demonstrate that VILK is equivalent to SE-ILK in its biological activities and can now be produced in large quantities by in vitro tissue culture.

Last Modified: 7/30/2014
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