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United States Department of Agriculture

Agricultural Research Service

Title: Reticuloendotheliosis Virus-Transformed Chicken T Cell Line Produces Lymphokines Which Confer Resistance to Se Organ Invasion in Day-Old Turkey Poults

Authors
item Genovese, Kenneth - TEXAS A&M UNIVERSITY
item Moyes, Rita
item Bowden, Lacy - TEXAS A&M UNIVERSITY
item Lowry, Virginia - TEXAS A&M UNIVERSITY
item Deloach, John
item KOGUT, MICHAEL

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 1, 1996
Publication Date: N/A

Technical Abstract: Our laboratory has previously reported an increased resistance to Salmonella enteriditis (SE) organ infection in chicks and turkey poults injected prophylactically with Salmonella enteriditis-immune lymphokines (SE-ILK) harvested from splenic T cells made from SE immune hens. In the present study, Con-A activated splenic T cells from SE hyperimmune hens were transformed in vitro using Reticuloendotheliosis Virus Strain T (REV- T) (chicken syncitial virus [CSV]). These transformed T cells were then maintained as a long-term cell line used for the harvest of SE-immune lymphokines. These viral-transformed T cell SE immune lymphokines (VILK) were then filtered and concentrated using Amicon YM100 and YM10 filters, respectively. The YM10 ultraretentate was collected and stored at -20 C. The efficacy of (VILK) to protect turkey poults against SE organ invasion was then evaluated. Three groups of day-of-hatch turkey poults were injected intraperitoneally with either 0.5 ml of VILK, 1.0 ml of SE-ILK, or 0.5 ml of sterile saline. Thirty minutes post-injection, poults were challenged per os with 1 x 10**5 cfu of SE. Twenty-four hours post- challenge, poults were euthanized and liver samples were cultured for the presence of SE. The results of those cultures were as follows (# SE positive/total): Trial 1: Control (27/33), VILK (7/32), SE-ILK (5/34); Trial 2: Control (20/20), VILK (10/23); Trial 3 Control (11/19); VILK (1/20). These results show that the virally-transformed T cell line produced lymphokines that were equally protective for turkey poults as the bird-derived SE-ILK.

Last Modified: 9/29/2014
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