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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Soybean Cultivars for in Vitro Culture of Soybean Cyst Nematodes

Authors
item Meyer, Susan
item Chitwood, David

Submitted to: Nematropica
Publication Type: Abstract Only
Publication Acceptance Date: July 7, 1996
Publication Date: N/A

Technical Abstract: Nematodes free of contaminating microorganisms can be produced in root explant culture and used for research purposes. Greater numbers of soybean cyst nematodes (Heterodera glycines) are produced on some susceptible soybean cultivars than on others, but there has been no quantitative enumeration of nematode populations on different cultivars. Consequently, 24 soybean (Glycine max) cultivars representing maturity groups 0-8 were tested with one isolate of H. glycines race 3 to determine which cultivar would produce the highest nematode populations. Excised soybean root tips were grown on Gamborg's B-5 medium (two root tips per petri dish). When the root tips were two days old, ten females (with attached egg masses) were inoculated onto each petri dish and incubated at 28.8 C. After 35 days, the total female and cyst population (excluding inoculum) was counted from each of ten petri dishes. The experiment was repeated once for each cultivar. The highest numbers of females and cysts were produced on cv. Bass (mean of 148 females and cysts per petri dish) and the lowest numbers on cv. Chesapeake (mean = 32). Nematode population numbers were not affected by maturity group. For example, in maturity group 3, Bass differed significantly from Pioneer 9392 (mean = 39). Due to the labor-intensive nature of rearing uncontaminated nematodes, this research demonstrates that it is worthwhile to test efficacy of cultivars, since populations can be more than quadrupled by cultivar selection.

Last Modified: 10/30/2014
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