|Jin, W - ISU|
|Horner, H - ISU|
Submitted to: Iowa Academy of Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: March 7, 1996
Publication Date: N/A
Technical Abstract: Ultrastructural abnormalities between normal and male-sterile (mutant) anthers were evident during late meiosis. Mutant tapetal cell cytoplasm became disorganized and highly vacuolate; many organelles were disrupted and ER profiles increased. Plastids in mutant cytoplasm of microspore tetrads developed dense bodies and surrounding callose was not degraded, in contrast to normal tetrads where it dissolved. Mutant tetrad microspores aborted while encased in callose. Southern blots showed that callase gene is present in both normal and mutant lines. Northern blots indicated a reduction in callase mRNA in mutant line, which suggests transcription may be affected. Further studies are being conducted to determine immunologically where callase is specifically produced in the normal line and whether translation and/or post-translation are/is affected.