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United States Department of Agriculture

Agricultural Research Service

Title: Identification of Blueberry Cultivars and Evaluation of Their Genetic Relationships Using Randomly Amplified Polymorphic DNA (Rapd) and Simple Sequence Repeat (Ssr) Primers

Authors
item Levi, Amnon
item Rowland, Lisa

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: September 27, 1996
Publication Date: N/A

Technical Abstract: Fifteen highbush blueberry cultivars (Vaccinium corymbosum Linnaeus), two rabbiteye blueberry cultivars (V. ashei Reade) and one southern lowbush (V. darrowi Camp) selection from the wild, were examined using seventeen 10-base (RAPD) and seven 17-18-base (SSR) primers in PCR reactions. Various levels of polymorphism were detected that readily distinguished between genotypes. Calculation of similarity values based on 131 and 52 distinct RAPD and SSR bands (respectively) revealed that among the V. corymbosum cultivars 'Weymouth' was the most divergent, while 'Sunrise' was the least divergent sharing relatively a large number of DNA fragments with most cultivars. The V. ashei cultivars and V. darrowi selection grouped out separately from the V. corymbosum cultivars as expected. Estimates of relative genetic distance between genotypes was compatible with known pedigree data, indicating consanguinity among few of the highbush (V. corymbosum) cultivars

Last Modified: 8/30/2014
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