Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Proceedings
Publication Acceptance Date: January 15, 1996
Publication Date: N/A
Normal healthy male volunteers (n=10) were fed diets containing 1.84 g/d of arachidonic acid (AA) for 50 days. The control diet contained 340 mg/d of AA. Details of the study protocol were given in an earlier abstract. Blood for fatty acid analyses was drawn from the antecubital vein after an overnight fast into syringes containing dextrose-citrate anticoagulant and separated into plasma, platelets and red blood cells. Adipose tissue samples (buttocks) were taken by needle biopsy, transferred to cold normal saline and frozen at -70 degree C until extraction. The samples were extracted with CHCl3-MeOH (2/1). The fatty acid composition of the tissues was determined by capillary gas-liquid chromatography (GC). Identities of the fatty acid esters were confirmed by GC-mass-spectroscopy. Lipoproteins, apolipoproteins, and cholesterol determinations were performed using an automated centrifugal analyzer on plasma drawn into a vacutainer containing EDTA. Dietary AA had no statistical significant effect on the blood cholesterol levels, lipoprotein distribution or apoprotein levels. Adipose tissue fatty acid composition was not influenced by arachidonic acid feeding The plasma total fatty acid composition was markedly enriched in arachidonic acid after fifty days (P<0.005). The fatty acid composition of plasma lipid fractions, cholesterol esters, triglycerides, free fatty acids, and phospholipid (PL), showed marked differences in the degree of enrichment in arachidonic acid. The PL plasma fraction from the subjects consuming the low-AA diet containing 10.3% AA while the subjects who consumed the high-AA diet had plasma PL fractions containing 19.0% AA.