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United States Department of Agriculture

Agricultural Research Service

Title: AFEX PRETREATMENT, ENZYMATIC HYDROLYSIS AND FERMENTATION OF GLUCOSE AND XYLOSE FROM CORN FIBER BY SACCHAROMYCES STRAIN (1400PLNH32)

Authors
item Moniruzzaman, Mohammed - TEXAS A&M UNIV
item Dale, Bruce - TEXAS A&M UNIV
item DIEN, BRUCE
item Chen, Z - PURDUE UNIV
item Ho, Nancy - PURDUE UNIV
item Hespell, Robert
item Bothast, Rodney

Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 9, 1996
Publication Date: N/A

Technical Abstract: Corn fiber is a grain processing residue containing significant amounts of cellulose, hemicellulose and starch which is collected in corn wet milling facilities where fuel ethanol is currently manufactured. Pretreatment of high moisture corn fiber by AFEX and fermentation of an enzyme hydrolyzate of mixed sugars by a recombinant Saccharomyces strain (1400pLNH32) were investigated. Approximate optimal pretreatment conditions for unground corn fiber containing 150% (dwb) moisture were: temperature, 90 deg C; ammonia:dry corn fiber mass ratio, 1:1; and residence time, 30 min. More than 85% of the theoretical sugar yield was obtained during enzymatic hydrolysis (48 h) after pretreatment of corn fiber under optimized conditions. The recombinant Saccharomyces strain (1400pLNH32) efficiently (91% theoretical yield) fermented both glucose and xylose contained in the corn fiber enzyme hydrolyzate and produced about 22 g/l ethanol.

Last Modified: 8/27/2014