Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 28, 1996
Publication Date: N/A
Technical Abstract: The search for lower cost raw materials for the production of biofuel has led to increasing interest in the enzymatic hydrolysis of cellulose to glucose. The enzyme, beta-glucosidase, is sensitive to glucose and is rate limiting during enzymatic hydrolysis of cellulose to glucose for the subsequent production of fuel alcohol. Candida peltata (NRRL Y-6888) produced a novel highly glucose tolerant beta-glucosidase. The enzyme was purified 1800 fold to homogeneity. The beta-glucosidase was a glycoprotein with molecular weight of 43,000. It displayed optimal temperature and pH at 50 deg C and 5.0, respectively, and had a specific activity of 108 units/mg protein. The beta-glucosidase was highly active against p-nitrophenyl-beta-D-glucoside, cellobiose and a series of higher cellooligosaccharides. It was highly tolerant to glucose with a Ki value of 1.4 M glucose. The biochemical and kinetic properties of the enzyme, mode of action and its use in the conversion of cellobiose and cellulose to glucose will be described.