Submitted to: Mammalian Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 12, 1995
Publication Date: N/A
Technical Abstract: The ob gene product is expressed in fat cells and has negative effect on appetite. Thus, an OB protein presumably has a central role in regulating body fat. We have used a cDNA clone of the rat ob gene to map on bovine homologue to chromosome 4 using RFLP analysis. Digestion on bovine genomic DNA with Bgl II resulted in polymorphic bands at 8.2 and 10.5 kb. Fifty percent of reference family parents were heterozygous for these bands. This is a first step in our efforts to characterize the role of OB protein in controlling appetite and fat deposition in livestock.