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United States Department of Agriculture

Agricultural Research Service

Title: Physical and Linkage Mapping of the Bovine Genome with Cosmids

Authors
item Hawkins, Gregory - AM. BREEDERS SERVICE
item Solinas-Toldo, S - ETH, ZURICH, SWITZERLAND
item Bishop, Michael - AM. BREEDERS SERVICE
item Kappes, Steven
item Fries, Ruedi - ETH, ZURICH, SWITZERLAND
item Beattie, Craig

Submitted to: Mammalian Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 19, 1994
Publication Date: N/A

Interpretive Summary: We report the physical assignment of ten bovine cosmid clones containing microsatellites to seven autosomes (5, 9, 13, 24, 25, 28, and 29). Eight microsatellites were informative (autosomes 5, 9, 13, 25, 28, and 29), allowing integration of genetic linkage and cytogenetic data. Furthermore, we demonstrate the general use of informative bovine cosmid clones to orient linkage groups spanning individual bovine chromosomes and physically anchor a comprehensive map for cattle.

Technical Abstract: We have initiated a mapping strategy using cosmid clones to chromosomally anchor a high-resolution bovine genetic linkage map. Ten cosmids containing microsatellites were assigned to bovine chromosomes by fluorescence in situ suppression hybridization (FISH). Four cosmid clones, three of which contain an informative microsatellite, were assigned to autosomes 5, 13, 24, and 28. The assignment to autosome 13 anchors bovine syntenic group U11. Two additional cosmid clones, each containing informative microsatellites, are assigned to autosomes 9 and 29, anchoring bovine linkage groups U2 and U8, respectively. Four cosmid clones, three of which contain informative microsatellites, also provide the first assignment to autosome 25, anchoring bovine syntenic group U7 and orienting the corresponding linkage group relative to the centromere.

Last Modified: 11/25/2014
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