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United States Department of Agriculture

Agricultural Research Service

Title: Directed Integration of the Physical and Genetic Linkage Maps

item Smith, Timothy

Submitted to: Swine Chromosome 7 Workshop
Publication Type: Proceedings
Publication Acceptance Date: September 15, 1995
Publication Date: N/A

Technical Abstract: The large scale generation of random microsatellite markers for the porcine genome is drawing to a close. With this skeletal map in hand, we can now proceed to identify areas of the genome for which it is desirable and/or necessary to increase marker density or fill in large intervals. Currently available technologies of chromosome microdissection and generation of chromosome segment-specific libraries permit this approach if the linkage and cytogenetic maps are sufficiently integrated. Integration can be rapidly achieved by a directed isolation of mapped markers on DNA segments of sufficient size for in situ hybridization. Application of an iterative screening process to isolate cosmids carrying SSC7 markers has produced a highly integrated physical and genetic linkage map of this swine chromosome. We demonstrate that the linkage map encompasses the entire chromosome, with significant variation of recombination rate as a function of position. In addition, FISH analysis of SLA markers reveals that the complex spans the centromere.

Last Modified: 4/21/2015
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