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United States Department of Agriculture

Agricultural Research Service

Title: Identification of Brucella Species by Pcr of Chromosomal Loci Containing the Repetitive Element Is711

Authors
item Bricker, Betsy
item Halling, Shirley

Submitted to: American Society of Microbiologists Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 12, 1995
Publication Date: N/A

Technical Abstract: Brucellae are gram-negative bacteria pathogenic to several economically important animals and to man. The six reported species of Brucella are nearly identical by RFLP maping. Current bacteriological methods for identification of the Brucella species require at least a week to perform. Previously we described a repetitive element, IS711, which is unique to the genus Brucella. We have developed a PCR assay which identified Brucella species based on the differential distribution of IS711 copies in each genome.

Last Modified: 4/18/2014
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