Submitted to: Journal of the American Oil Chemists' Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 16, 1996
Publication Date: N/A
Interpretive Summary: Surplus vegetable oils represent attractive renewable resources for the production of useful chemicals. We are investigating microbial conversion of vegetable oils and their component fatty acids to value-added products. A newly isolated microbial strain Clavibacterium sp. ALA2 converted unsaturated fatty acids from soybean oil to value-added oxygen containing product.
Technical Abstract: A novel compound, 12,13,17-trihydroxy-9(Z)-octadecenoic acid (THOA) was produced from linoleic acid by microbial transformation at 25% yield. The newly isolated microbial strain catalyzing this transformation was identified as Clavibacter sp. ALA2. The product was purified by high pressure liquid chromatography and its structure was determined by 1H and 13C nuclear magnetic resonance, Fourier transform infrared and mass spectroscopy. Maximum production of THOA was reached after 85 h of reaction. THOA was not further metabolized by strain ALA2. This is the first report on 12,13,17-trihydroxy unsaturated fatty acid and its production by microbial transformation.