|Galvin, Rachelle - LILLY, INDIANAPOLIS, IN|
Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: October 6, 1995
Publication Date: N/A
Technical Abstract: Pasteurella multocida type D toxin (PMT) causes progressive turbinate atrophy (atrophic rhinitis) in pigs. The mechanism of action of the toxin on bone cells on the turbinate is not clear, but effects on osteoclasts and osteoblasts have been implicated. This study examined the effects of PMT on osteoclasts differentiated from porcine bone marrow. The technique involves growing porcine bone marrow cells in the presence of Vitamin D3. By day 11 of culture, supporting stromal cells slough, leaving adherent multinucleated cells that express osteoclastic features, including positive staining for tartrate resistant acid phosphatase (TRAP) and the ability to resorb devitalized bone. PMT added to osteoclast culture media from days 1-11 increased the final number of TRAP + osteoclasts when compared to control cultures. Osteoclasts differentiated in the presence of PMT retained the ability to resorb bone and their TRAP + characteristics. This sstudy shows that PMT enhances the differentiation of osteoclasts in vitro. PMT may have similar effects on osteoclasts in vivo.