Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 14, 1995
Publication Date: N/A
Technical Abstract: Ruminant pestiviruses, bovine viral diarrhea virus (BVDV) and border disease virus (BDV) of sheep, are commonly classified based on the species from which they are isolated. Recently, two antigenically and genomically distinct subgroups of BVDV have been reported (BVDV 1 and BVDV 2). The relationship of the two BVDV genotypes to putative isolates of BDV is relatively unexplored. To study the phylogenic relationship of ruminant pestiviruses present in the United States, we obtained partial nucleic acid sequences for viruses isolated from cattle, goats, sheep, and llamas. Based on comparison of sequences from the 5' untranslated region, the viruses were segregated into three subgroups. As anticipated, two subgroups were represented by isolates of BVDV 1 and BVDV 2. The third subgroup contained viruses that were similar to BD-31, the border disease- type species available from the ATCC. All three subgroups contained both cytopathic and noncytopathic viruses. Members of these three subgroups could be differentiated by virus neutralization using polyclonal sera and by monoclonal antibody binding. Comparison of derived amino acid sequences from representative viruses of the subgroups revealed less than a 70% identity in the genomic region coding for viral structural proteins.