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United States Department of Agriculture

Agricultural Research Service

Title: Efficacy of Salmonella Enteritidis-Immune Lymphokines from Chickens and Turkeys on Se Organ Invasions in Day-Old Chicks and Turkey Poults

Authors
item Ziprin, Richard
item Kogut, Michael
item Mcgruder, Edward - TEXAS A&M UNIV.
item Hargis, Billy
item Corrier, Donald
item Deloach, John

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 19, 1995
Publication Date: N/A

Interpretive Summary: People in the United States are at continual risk of contracting food poisoning from the presence of the bacterium Salmonella in poultry products. As part of an effort to find a way to reduce Salmonella on live poultry we examined the effect of certain molecules (named immune lymphokines) on the ability of Salmonella cells to survive in newly hatched chicks and turkeys. We found that immune lymphokines produced by chickens will partially protect newly hatched turkeys from Salmonella, and that immune lymphokines produced in turkeys will partially protect newly hatched chicks. These observations indicate that it may ultimately be possible to manufacture one product useful for protecting both newly hatched chicks and turkeys.

Technical Abstract: We have previously shown that increased resistance to Salmonella enteritidis (SE) organ infectivity in day-old chicks was conferred by the immunoprophylactic administration of Salmonella enteritidis-immune lymphokines. These lymphokines have previously been found to be present in the cell culture media of comcanavalin A stimulated splenic lymphocytes obtained from SE immunized chickens. In the present study we evaluated whether turkeys also produced SE-immune lymphokines (SEILK), and whether these lymphokines could protect day-old chicks and turkey poults against SE organ invasion. In addition, we tested the ability of our original chicken SEILK to reduce SE organ invasion in turkey poults. Day-of-hatch chicks and turkey poults were infected intraperitoneally with immune lymphokines of either chicken or turkey origin. One hour later the birds were challenged per os with SE and 20 hours later their livers were examined by bacteriological methods for the presence of SE. We found that SE immune lymphokines induced from the splenic lymphocytes of SE immunized turkeys reduced SE organ invasion in both chicks and turkey poults. Conversely, we also determined that SE-immune lymphokines produce by chicken splenic lymphocytes conferred protection against invasion by SE in turkey poults. This research is the first report of the production of SE-immune lymphokine in turkeys and also the first report on the cross species activity of these effector molecules in these poultry species.

Last Modified: 10/22/2014
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