ARS | Publication request: UMC MAIZE RFLP PROCEDURES MANUAL

Submitted to: Restriction Fragment Length Polymorphism Procedure Manual U Missouri Columb
Publication Type: Book / Chapter
Publication Acceptance Date: August 30, 1995
Publication Date: N/A

Technical Abstract: Following numerous inquiries from other researchers for the procedures we utilize in developing the public UMC Maize RFLP map, we have compiled our current protocols in an easily distributed form. Procedures outlined in the UMC Maize RFLP Procedures Manual represent those currently in use or those which have been used successfully in the Maize RFLP Laboratory at the eUniversity of Missouri. While there are probably as many ways to isolate plant genomic DNA and to transfer DNA from a gel to a membrane as there are researchers in the area, these procedures have been used routinely and appear to be very dependable in the RFLP analysis of the various plant species studied in the lab. While these procedures basically are those in use as of the time of this revision, we are constantly evaluating various modifications to each of the steps, particularly if they would result in significant cost or time savings. An electronic version of this manual with indexing is available on the Maize Genome Database front page in the World Wide Web (http://www.agron.missouri.edu).