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United States Department of Agriculture

Agricultural Research Service

Title: Barley Seed Alpha-Glucosidases: Their Characteristics and Roles in Starch Degradation

Authors
item HENSON, CYNTHIA
item Im, Hana - UNIV OF WI

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: August 12, 1996
Publication Date: N/A

Technical Abstract: Starch in germinating cereals seeds is degraded by alpha-amylase, beta-amylase, debranching enzyme and alpha-glucosidase . It has long been accepted that alpha-amylase is the most important enzyme in starch degradation. Recent studies identified alpha-glucosidase as the second most important enzyme. They also showed that it can initiate native starch hydrolysis and established that it is critical for subsequent metabolism of starch breakdown products. A reconstituted system was used to establish the ratio of alpha-glucosidase/alpha-amylase that results in maximal synergy in the degradation of native starch. 3H-conduritol B epoxide (CBE), a specific alpha-glucosidase inhibitor was used to label the active site of barley alpha-glucosidases. Two isoforms were identified by CBE labelling and by size exclusion chromatography.

Last Modified: 7/25/2014
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