Submitted to: Rushmore Conference on Mechanisms in Pathogenesis of Enteric Diseases
Publication Type: Abstract Only
Publication Acceptance Date: September 28, 1995
Publication Date: N/A
Edema disease in weaned pigs is a degenerative angiopathy caused by Shiga-like toxin II variant-producing E. coli. F107 is the fimbrial adhesin that facilitates adherence to specific receptors in the pig's small intestine. Pigs lacking receptors are not colonized by F107+ E. coli and are resistant to edema disease. The porcine gene that controls resistance or susceptibility to colonization is on chromosome 6. However, the F107 receptor has not been identified. An F107-binding immunoassay (F107-BIA) was developed to assess the presence of F107 receptors in the small intestine of pigs. The F107-BIA, unlike the microscopic adhesion assay (MAA), does not require intact brush borders to detect intestinal receptors. Brush border fragments were fixed on membranes and overlaid with F107. Bound F107 was detected by an indirect immunoperoxidase assay. The MAA and F107-BIA correlated well in a selected litter (n=8) and the results agreed with the F107 phenotype predicted on the basis of genetic markers on chromosome 6. The genes encoding for porcine stress syndrome and F107 binding segregated in this litter. The F107-BIA will be useful for identification and characterization of porcine intestinal F107 receptors.