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United States Department of Agriculture

Agricultural Research Service

Title: Short Communication-Porcine Sine Associated Microsatellite Markers: Evidence for New Artiodactyl Sines

Authors
item Alexander, Leeson
item Rohrer, Gary
item Stone, Roger
item Beattie, Craig

Submitted to: Mammalian Genome
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 1995
Publication Date: N/A

Technical Abstract: Approximately 24% (170/710) of porcine (dG-dT)n.(dC-dA)n microsatellites isolated in our laboratory are associated with a previously described porcine Short Interdispersed Element (SINE) termed PRE-1 SINE. Another 5.6% (40/710) of the microsatellites were adjacent to two previously unidentified SINE sequences which we have designated ARE-1P (Artiodactyl Repetitive Element-1 Porcine) and ARE-2P. The ARE repeats were also found in bovine microsatellite and genomic sequences in the GenBank database. Genotypic information was obtained from 68.9% of primers where at least one primer sequence was obtained from the PRE-1 SINE and 66.6% of primer pairs designed from the ARE SINEs. The use of primers derived from SINEs significantly increases the number of primer pairs available for genetic linkage studies in swine

Last Modified: 8/22/2014