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United States Department of Agriculture

Agricultural Research Service

Title: Lymphocyte Proliferation in Response to Brucella Abortus Rb51 and 2308 Proteins in Rb51-Vaccinated Or 2308-Infected Cattle

item Stevens, Mark
item Olsen, Steven
item Cheville, Norman

Submitted to: Infection and Immunity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 7, 1995
Publication Date: N/A

Interpretive Summary: Cattle are currently given the Brucella abortus strain 19 vaccine to prevent brucellosis and abortions that are caused by the bacteria, B. abortus. Vaccination with strain 19 and testing cattle for brucellosis has almost eradicated brucellosis from cattle in the United States. The B. abortus strain RB51 vaccine is being tested to replace the strain 19 vaccine. Strain RB51 is better than strain 19 as a vaccine because cattle having brucellosis can be more easily identified and removed from vaccinated herds. Therefore, eradication of brucellosis from cattle may be more rapidly achieved by using the RB51 vaccine. Results from this study indicated the RB51 vaccine stimulates certain immune responses in cattle which are important in combating brucellosis. These findings further support the use of the RB51 vaccine in preventing brucellosis in cattle.

Technical Abstract: Cattle vaccinated with Brucella abortus strain RB51 (SRB51) or infected with strain 2308 (S2308) had lymph node lymphocytes which proliferated most when incubated with 32, 27, 18, or <18 kDa proteins of either SRB51 or S2308. Some S2308-infected cattle but no SRB51- vaccinated cattle had lymphocytes which proliferated in response to 80 and 49 kDa proteins of SRB51 and S2308. These results suggest that cattle vaccinated with SRB51 or infected with S2308 have lymphocytes which proliferate in response to most of the same S2308 proteins and that the immunodominant protein antigens of SRB51 and S2308 have similar molecular masses of 32, 27, 18, and <18 kDa.

Last Modified: 4/18/2015
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