ARS | Publication request: UTILITY OF MAPPED PROBES FOR DETECTING DIFFERENCES BETWEEN 28 NORTH AMERICAN BARLEY CULITVARS.

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 7, 1995
Publication Date: N/A

Technical Abstract: Genetic linkage maps of molecular markers have been developed for many crop species including barley (Hordeum vulgare L.). One of the goals of the North American Barley Genome Mapping Project (NABGMP) is to use our RFLP map to aid in release of improved barley cultivars. The objective of this study was to determine the range of variation or polymorphism information content (PIC) detected at RFLP marker loci among 28 barley cultivars. DNA extracted from these cultivars was cut with BamHI and hybridized to sequences mapped by the NABGMP. Approximately 40% of the probe sequences did not identify any polymorphism on DNA cut with BamH1, giving a PIC of zero. The other probes identified two to nine different banding patterns across the 28 cultivars tested. The PICs for these probes ranged from 0.069 to 0.818. The overall average PIC for all probes tested was 0.274, while the average PIC for probes that identified polymorphisms was 0.466. Probes that identified polymorphisms could be found for most pairs of cultivars.