ARS | Publication request: SOYBASE 1995: NODULINS AND NITROGEN REDUCTION

Submitted to: Nitrogen Fixation International Congress
Publication Type: Proceedings
Publication Acceptance Date: July 7, 1995
Publication Date: N/A

Technical Abstract: SOYBASE, a soybean genome database, now contains approximately 100 metabytes of data. It employs a user-friendly, "point and click" hypertext system. SOYBASE recently was expanded to incorporate metabolic data. Forty-one pathways of nitrogen and fatty acid synthesis and degradation are diagrammed, revealing the respective enzymes, reactants, products, cofactors, kinetics, inhibition, activation, and regulation. More than 200 individual enzymes are detailed. Tags include EC number, gene symbol, purification, physical properties, source material, existence and accessibility of mutants, isozymes, sequence data, transgenic plants, and availability of DNA or antibody probes. Interconnections link pathways with enzymes. Furthermore, the entire database is interlinked through bold-faced, "clickable" hypertext items within any topic. Thirty-seven nodulins of soybean are summarized, showing related nodulins and sequences, degree of conservation, source, bacterium, time of initiation, possible function, physical characteristics, and availability of probes and transgenic plants. Additional material in the database includes 3 molecular RFLP maps, 1 classical map, 2-point genetic data, RFLP probes and images of autoradiograms, pathology data, storage proteins, and QTL studies. SOYBASE contains approximately 20,000 germplasm records with available trait data. Nitrate and nitrite reduction and their respective enzymes are currently part of SOYBASE, as are the synthesis and degradation of ureides. The database will be expanded to include biological nitrogen fixation, since this process distinguishes legumes from other crops.