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United States Department of Agriculture

Agricultural Research Service

Title: A Technique to Semi-Quantitate Leukocyte Passage into Lung

Authors
item Ackermann, Mark
item Kehrli Jr, Marcus
item Brogden, Kim

Submitted to: American College of Veterinary Pathologists Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 31, 1995
Publication Date: N/A

Technical Abstract: Passage of CD18- and CD18+ neutrophils through the septa of pulmonary alveoli was measured in 3 cattle with CD18- deficiency and 4 normal (CD18+) cattle. Cattle received Pasteurella haemolytica A1 by bronchoscopy; control lobes received PFS. Alveolar lumens were delineated (anti-cytokeratin), and pulmonary macrophages were stained (anti-CD68) in a double-labeling technique. Parameters measured by computerized imaging (VIDAS 2.1) included alveolar lumen area, area of alveolar septa, number of intraluminal neutrophils, number of intraseptal neutrophils, and number of intraluminal and intraseptal macrophages. Increased numbers of neutrophils and macrophages were present in the alveolar septa of PFS (control) lobes of CD18- cattle when compared to CD18+ cattle. Increased numbers of neutrophils (7.9 +/ 1.2) were present in the alveolar septa of P. haemolytica- inoculated lobes of CD18- cattle when compared to CD18+ cattle (2.1 +/ 0.7). This technique was repeated on 6 cattle and 6 sheep that were inoculated with P. haemolytica, LPS, or LPS-Associated Protein (LAP) and necropsied 48 hrs PI. LAP-inoculated lobes had increased numbers of macrophages in comparison to bacteria and LPS inoculae. This technique is useful for measuring infiltration of inflammatory cells into alveolar lumen of lung in both cattle and sheep.

Last Modified: 10/21/2014
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