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United States Department of Agriculture

Agricultural Research Service

Title: Comparative Evaluation of Three Methods for Detection of Reticuloendotheliosis Virus As a Contaminant in a Live-Virus Vaccine of Poultry

Authors
item Fadly, Aly
item Witter, Richard

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 12, 1995
Publication Date: N/A

Technical Abstract: Indirect immunofluorescence (IFA), polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were evaluated as methods for detection of reticuloendotheliosis virus (REV) as a contaminant in a live-virus vaccine of poultry. IFA and PCR were more sensitive than ELISA in detection of REV in chicken embryo fibroblasts (CEFs) inoculated with REV-contaminated vaccine. However, when the vaccine was tested in chickens, the sensitivity of ELISA was comparable to that of IFA and PCR. Filtration of vaccine before testing in CEFs, to rid inoculum of vaccine virus, resulted in significant reduction in frequency of detecting REV by PCR or IFA. The data also suggest that the sensitivity of all three tests depends on the concentration of REV in the vaccine.

Last Modified: 9/1/2014
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