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United States Department of Agriculture

Agricultural Research Service

Title: Enzyme-Linked Immunosorbent Assay (Elisa) for Pastueuria Nishizawae

item Reise, Robert
item Chitwood, David
item Hackett, Kevin
item Sayre, Robert
item Huettel, Robin

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: April 19, 1995
Publication Date: N/A

Technical Abstract: An ELISA-based procedure for quantifying the number of Pasteuria nishizawae spores was developed with a monoclonal antibody generated in mice against a homogenate of P. nishizawae. The method could detect P. nishizawae obtained from Heterodera glycines cysts in concentrations as low as 1,600 spores/ml. Pasteuria penetrans obtained from Meloidogyne arenaria also produced a strong but somewhat weaker response in the ELISA. Absorbance values obtained during the procedure were near zero for samples of homogenates of uninfected H. glycines or M. arenaria. The other bacterial species examined (Bacillus thuringiensis, Streptomyces spp., and Escherichia coli) failed to react with the antibody. A few problems with nonspecific activity associated with endogenous peroxidases in plant root samples were observed but were overcome by procedural modifications

Last Modified: 4/18/2015