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United States Department of Agriculture

Agricultural Research Service

Title: Characterization of Acetyl-Coa Carboxylase Genes in Soybean

Authors
item Anderson, James
item Gronwald, John
item Plaisance, Kathryn - UNIVERSITY OF MINNESOTA
item Gengenbach, Burle - UNIVERSITY OF MINNESOTA

Submitted to: National Plant Lipid Cooperative Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 1995
Publication Date: N/A

Technical Abstract: Acetyl-CoA carboxylase (ACCase) catalyzes the ATP-dependent carboxylation of acetyl-CoA to form malonyl-CoA. In soybean we have characterized two > 14 kb nuclear-encoded ACCase genes (ACCase-A and B) and one 330 bp PCR amplified ACCase fragment from chloroplast DNA. ACCase-A is encoded by 6789 nucleotides in 31 exons. Compared to ACCase-A, the ACCase-B gene is missing g849 bp of coding sequence at its 3' end. Sequence data from neither gene has indicated the presence of a chloroplast transit peptide. The 330-bp PCR fragment shows > 85% sequence homology to the carboxybiotin binding domain of the accD gene which encodes a subunit of the prokaryotic-type ACCase. SDS-PAGE analysis of two ACCase fractions isolated from soybean embryos provides evidence for multiple forms of ACCase. One ACCase fraction contained a 210-220 kDa biotinylated polypeptide whereas the other ACCase fraction contained only lower molecular mass biotinylated polypeptides including a 35 kda band which may represent the BCCP subunit of the prokaryotic-type ACCase. We are using DNA probes to ACCase to determine its expression in soybean.

Last Modified: 10/23/2014
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