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United States Department of Agriculture

Agricultural Research Service

Title: Sudden Death Syndrome of Soybean: Further Characterization and Purification of the Toxin Produced by Fusarium Solani

item Jin, Hua - UNIV OF ILLINOIS
item Hartman, Glen
item Widholm, Jack - UNIV OF ILLINOIS

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: October 3, 1995
Publication Date: N/A

Technical Abstract: A 17 kD phytotoxic polypeptide was identified from culture filtrates of Fusarium solani the causal agent of soybean sudden death syndrome. The phytotoxoin was heat unstable, negatively charged, absorbed by 10% charcoal and destroyed by proteinase K. Purification of the phytotoxin was achieved by gel filtration of Sephadex G-50 chromatography followed by ion exchange chromatography on a DE-52 column. The purified protein migrated as a single band on SDS polyacrylamide gels. The N-terminal 15 amino acids were sequenced. Samples containing this single protein caused browning on soybean calli, necrosis on detached soybean cotyledons, and caused yellowing, curling, and drying on intact cotyledons and leaves of seedlings.

Last Modified: 4/22/2015
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