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United States Department of Agriculture

Agricultural Research Service

Title: Silver Staining for Elucidation of the Synlophe in Trichostrongyle Nematodes

Authors
item Khrustalev, A - MOSCOW INST. HELMINTHIC
item Hoberg, Eric

Submitted to: Journal of Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 22, 1995
Publication Date: N/A

Interpretive Summary: Recent studies of the economically important trichostrongyloid nematodes have relied on examination of the synlophe. This attribute, a system of longitudinal cuticular ridges, is one of the most important characters for research in systematics. A novel silver staining method is described for elucidation of the synlophe. Ridges are stained optically black and appear in great contrast to the body of the nematode. This method augments current use of interference contrast for examination of the synlophe and makes it possible to conduct detailed studies with standard light microscopy for the first time. This technique provides an inexpensive and powerful tool in diagnostics and systematics of these nematodes.

Technical Abstract: Staining techniques are relatively rare in the study of parasitic nematodes. A novel silver staining method is described for elucidation of the synlophe (a system of longitudinal cuticular ridges), a character of great systematic importance among the trichostrongyloid nematodes. Ridges are stained optically black and appear in great contrast to the body of the nematode. This method augments current use of interference contrast for examination of the synlophe and makes it possible to conduct detailed studies with standard light microscopy for the first time.

Last Modified: 7/22/2014
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