ARS | Publication request: CHICKEN MHC ALLOANTISERA CROSS-REACTIVITIES AS DETECTED BY HEMAGGLUTINATIONAND FLOW CYTOMETRY

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 16, 1995
Publication Date: N/A

Technical Abstract: Chicken major histocompatibility B-complex alloantisera produced following immunization with erythrocytes (RBC) or whole blood cells were tested by hemagglutination (HA) of RBC and by indirect immunofluorescence using flow cytometry of RBC and peripheral blood lymphocytes (PBL). Birds used for both producing and testing the alloantisera were from the 15I5 B-congenic lines; thus antisera reactivities and cross-reactivities should reflect only B haplotype differences. A total of 51 alloantisera were examined for reactivities with cells from B**2, B**5, B**12, B**13, B**15, B**19 and B**21 homozygotes. HA and flow cytometry analysis of antibody reactivities with RBC showed no differences in these two methods for detection of B- haplotype specific antibody binding and cross-reactivities. Five cross- reactive RBC-specific epitopes were detected: B2:B21, B5:B19, B12:B19, B15:B21, and B19:B21. In addition, six cross-reactive epitopes were identified that were expressed on both RBC and PBL: B2:B12, B2:B15, B5:B21, B12:B13, B13:B19, and B15:B19. Knowledge of these cross- reactivities is beneficial for planning production of antisera, selecting antisera for determining MHC haplotype, and for predicting similarities of B-complex molecules.