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Title: USE OF THE POLYMERASE CHAIN REACTION FOR SALMONELLA DETECTION

Author
item Kwang, Hwei Sing
item Littledike, E
item Keen, James

Submitted to: Letters in Applied Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/13/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: This paper describes a gene amplification method (polymerase chain reaction, PCR) that detects Salmonella bacteria. This method detected all 40 Salmonella serovars (60 isolates), but not the non-Salmonella bacteria (42 isolates) that have been tested. The Salmonella serovars tested include the most frequently identified serotypes associated with disease in cattle, swine, humans, chickens, and turkeys. This test appeared to be highly specific for the in vitro detection of Salmonella organisms.

Technical Abstract: A primer set of oligonucleotides (S18 and S19) from the ompC gene of Salmonella has been evaluated for specific detection of Salm. by polymerase chain reaction (PCR). This primer set successfully amplified 40 Salm. serovars (60 isolates), but not 24 non-Salm. bacteria (42 isolates) that we have tested so far. The uniqueness of these primer sequences was also confirmed.