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United States Department of Agriculture

Agricultural Research Service

Title: Mechanical Transmission of Genomic Clones of Maize Streak Geminivirus

Authors
item Ngwira, P - OHIO STATE UNIV
item Louie, Raymond
item Njuguna, J - OHIO STATE UNIV
item Bisaro, D - OHIO STATE UNIV
item Gordon, Donald - OHIO STATE UNIV

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: March 31, 1995
Publication Date: N/A

Technical Abstract: Viral dsDNA (RF) was isolated from maize leaves infected with a Nigerian (MSV-N) or Kenyan (MSV-K) isolate of maize streak virus. The DNA was restricted with Bam HI and cloned into plasmid pGEM7 as a tandem duplication (MSV-K) or as a 1.5-mer containing two copies of the plus strand origin of replication (MSV-N). The infectivity of MSV DNA in the recombinant plasmids was tested by vascular puncture inoculation of Seneca Chief sweet corn seeds. In the first test, MSV-N and MSV-K were transmitted to plants from 0 of 50 and 23 of 49 inoculated seeds, respectively. In the second, these constructs were transmitted to plants from 3 of 96 and 24 of 98 inoculated seeds, respectively. Plants infected by MSV-N exhibited mild symptoms which appeared at 10 days after inoculation (d.a.i.), whereas plants infected by MSV-K exhibited severe symptoms at 7 d.a.i. Mostly the dsDNA RF was recovered from MSV-N infected plants, whereas larger amounts of all genomic DNA forms, including ssDNA, were recovered from MSV-K infected plants. This is the first report of mechanical transmission of cloned MSV DNA without the aid of biolistic delivery or agroinoculation. It is expected that this technique will greatly facilitate the genetic analysis of MSV.

Last Modified: 10/21/2014
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