|Solinas Toldo, Sabina - ETH, ZURICH|
|Fries, Ruedi - ETH, ZURICH|
Submitted to: DNA and Cell Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 11, 1996
Publication Date: N/A
Interpretive Summary: This study has been conducted to identify a gene called Fas/APO-1 which is known to determine the fate of cells; to die or to live. We isolated the Fas/APO-1 gene from cattle and identified the regions of the gene that is translated into the functional protein. There are 29 different chromosomes in cattle along with X and Y chromosomes. We showed that there is only one ecopy of the gene in entire cattle chromosomes. In addition, we used both genetic and physical methods to determine where in the cattle chromosomes the Fas/APO-1 gene is located and determined that this gene is on cattle chromosome 26. The present study will help to better understand the role of this gene in controlling the fate of cell populations that flourish to perform a function then die. Examples include cells in the ovary and immune cells in the thymus.
Technical Abstract: The cell-surface protein Fas (APO-1) is a member of the tumor necrosis factor receptor (TNFR) superfamily and transduces apoptosis following binding of Fas-ligand or exposure to certain anti-Fas (APO-1) antibodies. We have isolated the bovine Fas (APO-1) gene and determined its genomic organization. Genomic Southern hybridization analysis indicated Fas(APO-1) to be a single copy gene. DNA sequence comparison with the bovine Fas cDNA demonstrated that bovine Fas gene consists of 9 exons spanning approximately 31.5 kb. The 5'-flanking region is GC-rich, lacks conventional TATA and CCAAT elements, and contains several potential regulatory elements including multiple copies of Sp1, AP-2, E-box and N-boxes. Linkage analysis of two (CA) dinucleotide repeat microsatellites within intron 1 and physical assignment by fluorescence in situ hybridization (FISH) placed the Fas gene on bovine chromosome 26. The present data will help to better understand the transcriptional regulation of the Fas (APO-1) gene expression.